|
KMID : 0861020180330010037
|
|
Korea Journal of Herbology
2018 Volume.33 No. 1 p.37 ~ p.46
|
|
|
Protective Effect of Tongyuhwalhyeol-tang on Liver Injury in Thioacetamide-induced Rat
|
|
Kim Kyeong-Jo
Shin Mi-Rae
Kim Soo-Hyun
Kim Su-Ji
Lee Ah-Reum
Kwon Oh-Jun
Roh Seong-Soo
|
|
|
Abstract
|
|
|
Objectives : Liver disease is an inflammatory reaction caused by oxidative stress, viral, alcohol, and drug properties. Inflammatory reaction causes hepatitis and chronic hepatitis is persistent, it progresses to liver fibrogenesis and liver cancer. The aim of this study was to confirm the hepatoprotective effect of Tongyuhwalhyeol-tang(Tongqiaohuoxue Decoction) (TH) and Gamtongyuhawlhyeol-tang(GTH) in TAA-induced liver injury animal model.
Methods : The antioxidant activities were evaluated through in vitro experiments, such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2’-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays, total polyphenol and total flavonoid content measurement. To confirm the liver protective effect, induced by Thioacetamide (TAA) for 3 days injection at 200 ㎎/㎏ rats. TH and GTH were treated 3 days at 200 ㎎/㎏/day. The changes of reactive oxygen species (ROS), peroxynitrite (ONOO-), alanine aminotransferanse (ALT) and aspartate aminotransferase (AST) in serum were analyzed after experiment. Also, expression of anti-inflammation, anti-oxidant related proteins were investigated by western blot analysis.
Results : TH was inhibited the antioxidant activities. In the TAA-induced rat, TH decreased ROS, ONOO-, ALT, AST level in serum. Inflammation related protein expressions increased in TAA-induced rat compared to normal rat. However, TH group inhibited the down expression of these proteins. Also, anti-oxidant related protein expressions increased in TH group compared TAA-induced rat.
Conclusion : Therefor, these results suggested that TH provided hepatoprotective effects on the hepatic injury leading to the reduction of inflammatory response. In addition, the effect of TH was superior to that of GTH.
|
|
|
KEYWORD
|
|
|
Liver injury, hepatitis, tongyuhwalhyeol-tang (Tongqiaohuoxue Decoction), thioacetamide, reactive oxygen species, anti oxidant
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|